Thermal degradation of natural dyes and their analysis using. Biodegradation and decolourization of textile dye wastewater. Aug 08, 2017 decolourization and degradation of dyes in industrial effluents by native endophytic fungi dr. Biodegradation of dyes using consortium of bacterial strains. Its structural stability makes it highly toxic and resistant to biodegradation. Isolation and screening of dye degrading microorganisms. Bioremediation of dyes by fungi isolated from contaminated dye. Degradation of azo dyes by trametes villosa laccase over long. The result of the dye degradation by the isolate is shown in figure 1af.
Discoloration and biodegradation of two dyes by whiterot. Decolorization of azo, anthraquinone, heterocyclic, triphenylmethane and polymeric dyes by white rot fungus phanerochaete chrysosporium have been reported11,12. Results of the dye biodegradation by soil fungi in this study using spectrophotometric analysis were even comparable with the percent dye decolorization exhibited by the whiterot fungus trametes versicolor and pleurotus ostreatus yao et al. Bioremediation of dyes by fungi isolated from contaminated.
Degradation of azo dyes by trametes villosa laccase over. An analysis of the data reveals that out of four solid media used three did not show the degradation of dye by the growing microorganisms. The outcome of the study contributes in taking microbial dye remediation from laboratory to field conditions. Decolorization of reactive dyes by trametes villosa ccb176 and pycnoporus sanguineus ufmgcb03 on malt extract agar containing 0. Biodegradation of dyes by basidiomycetes fungi using hplc. Screening of fungi for decolorization of dye wastewater.
While fungal treatment of dye containing effluents is usually time consuming and difficult to control24, the potential of enzymes for this purpose has clearly been demonstrated. Degradation dyes lignindegrading fungus phanerochaete. Screening of soilderived fungi for dye decolorization activities. The outcome of the study contributes in taking microbial. The lignindegrading white rot fungus phanerochaete. Fungal degradation of the bioplastic phb poly3hydroxybutyric acid k. Chacko, kalidass subramaniam international journal of environmental sciences volume 1 no. Microbial degradation and decolourization of dyes in semi. With the same interest, present study focuses on exploration of dye degradation efficiency of three different pleurotus sp. Pdf bioremediation of dyes by fungi isolated from contaminated. Pdf biodegradation and detoxification of dyes, malachite green, nigrosin and basic fuchsin have been carried out using two fungal isolates. Isolation and screening of dye degrading microorganisms from.
The goal of fungal treatment is to decolorize and detoxify the dye contaminated effluents. Aspergillus niger recorded maximum decolorization of the dye basic fuchsin 81. Characterization of an azodyedegrading white rot fungus. Ligninolytic enzyme and dye degradation activity was tested against azo tex. This is not necessary if you use an aluminum, tin, or copper pot as the metal in the pot will take part in the dyeing reaction. All the media used differs in the capacity to support the growth of. Decolorization of dye containing wastewater by fungi.
Different bacterial isolates were isolated from activated sludge of common effluent treatment plant and tested against reactive violet 5 rv5 dye. Biodegradation of textile dyes, bromophenol blue and. Data is presented showing how various factors affect enzymatic azo dye degradation. Table 1 biodegradation of textile dyes, bromophenol blue and congo red by a. Jun, 2012 the uv spectrum confirms the decolourization. Several other dye decolorizing fungal species have been reported, which include aspergillus niveus 2 ve fusarium moniliforme 8. They were each mixed with 5 days old mycelia in roughly 2. Bacterial degradation of textile dyes springerlink. Discoloration and biodegradation of two dyes by whiterot fungi. This is not necessary if you use an aluminum, tin, or copper pot as.
Recently, several other white rot fungi have been shown to. Decolorization of triphenylmethane dyes by six whiterot fungi. Hence, it was concluded that ganoderma lucidum could be utilized for the effective treatment of textile dye wastewater. Fungal decolourization of direct azo dyes and biodegradation of textile dye effluentdecolourization degradation direct azo dyes textile dye effluent. Screening of fungi for decolorization of dye wastewater ipcbee. Singh and others published degradation of anthroquinone dyes stimulated by fungi find, read and cite all the research you need on researchgate. Substantial quantities of these dyes have been deposited in the environment, particularly in streams and rivers. All the media used differs in the capacity to support the growth of dye degrading microorganisms. In all cases, bacterial degradation is initiated bythe reduction ofthe azo corresponding author.
Fungal degradation of the bioplastic phb poly3hydroxy. Because these fungi grow into spheres in liquid culture, they can be counted to provide an. Dye removal from wastewater is a difficult task and expensive2. The extent of degradation for these dyes by fungi can be evaluated with the help of uvvisible spectrophotometer and high performance liquid chromatography. The isolated bacteria were capable of decolorizing 0. Analysis of bacterial degradation of azo dye congo red using hplc. Recent studies on the use of fungi and enzymes for bioremediation of azo dyes are surveyed. However, lasparagine was used as the original nitrogen source and it was known that amino acid could also be the source of carbon for fungi. Several medium factors can influence the ability of fungi in biodegradation, such as temperature, concentration of dye, ph and the chemical structure of dye. The fungi were also grown in culture medium without the synthetic effluent. Learners discover that natural chemicals in fungi can dye wool different colors.
Various models were tried to study the kinetics of textile dye degradation. Azo dyes are used in a wide variety of products and can be found in the effluent of most sewage treatment facilities. Therefore, the purpose of this work was the study of azo dye degradation products in the presence of laccase. Hplc analysis of the intermediates and end products from dye degradation showed that the decolorized dyes by the in vitro systems could still have residual fragments such as aromatic rings which had high uv adsorption. In this study we intended to isolate and identify azo dye degrading and decolorizing bacterial strains as well as investigation its biodegradation mechanism. Pdf degradation of anthroquinone dyes stimulated by fungi.
Introduction the two major sources of dye release into the environment are the textile and dyestuff manufacturing industries1. Biomass degradation refers to the utilization of microorganisms such as bacteria and fungi to produce enzymes that can interact with molecules of dyes. Degradation of azo dyes by environmental microorganisms and. White rot fungi were capable of secreting active ligninolytic enzymes and decolourising the pulp and paper mill. Biodegradation of reactive red m5b dye using bacillus subtilis. The ability to decolorize three triphenyl methane dyes crystal violet, bromophenol blue and. From the results, it was found that the degradation of textile dye wastewater follows firstorder kinetics. Decolorization pathways of anthraquinone dye disperse blue. The most potent isolate was identified as pseudomonas spp via partial sequencing of 16s. By using certain fungi, eco friendly degradation of azo dyes can be carried out to remove toxicity and thus can help in keeping the environment free from pollution.
A fixed amount of each dye was used in the culture medium to screen out the potential of the fungus for dye degradation. The objective of this study was to assess the congo red biodegradation and detoxification by aspergillus niger. Decolourization and degradation of dyes in industrial effluents by native endophytic fungi dr. Decolorization study showed that the methyl orange dye was removed by more than 50% in 3 days, eriochrome balck t removed by more than 33% and hplc analysis determined several degradation products. The retention times of the peaks of berberine 1, palmatine 2, alizarin 3, purpurin 4, indigotin 5, and indirubin. Among these basdiomycetes are the best producers of laccase. Table 2 decolourization of dye containing waste water using fungi. Several microorganisms, such as fungi, bacteria and yeasts, have ability to discolour and even completely transform many dyes under optimum conditions. Bioremediation takes place by anaerobic andor aerobic process. Effect of agitation on dye degradation the effect of agitation on dye degradation was determined by incubating msm broth containing fungi at various rpm revolution per minute like 20,40,60,80,100 for 24 hrs. According to our uv spectroscopic methods, biodegradation of dyes occur by the fungi used in the kirks medium.
Textile effluent, azo dye, congo red, decolourization, bio degradation, hplc. By using certain fungi, eco friendly degradation of azo dyes can be carried out to remove toxicity. These fungi have selected in present study because a. White rot fungi, coriolus versicolor, termetomyces sp, pleurotus ostreatus, schizophyllum commune, decolourization of azo dyes. Studies and research in zoology, scott christian college autonomous, nagercoil 629 003, kanyakumari district, tamil nadu, india key words. Joel gnanadoss et al bioremediation of textile dye using white rot fungi. The effects of ph, initial dye concentration, temperature, and shaking. Biodegradation of textile dyes, bromophenol blue and congored by fungus aspergillus flavus. Phenol kills the fungi, and the lactic acid increases preservation chino cotton blue is a stain for chitin and cellulose. The present study deals with the degradation of methyl red mr and carbol fuchsin cf by bacteria and its consortium isolated from textile effluents. Dye production in india is estimated to be around 64,000 tonnes, which is about 6.
The percent dye degradation by the test isolate at different ranges of. The inhibition of fungal growth in dyecontaining medium was also observed during dye degradation table 1. Congo red decolorization and detoxification by aspergillus. The capability of whiterot fungi to removal synthetic dye is due to extracellular enzyme production such as laccases, lignin peroxidases lip and manganese peroxidases mnp atteke et al. Analysis of bacterial degradation of azo dye congo red using hplc d. Screening of soilderived fungi for dye decolorization activities sixty one dye effluent soil fungal strains were screened for their ability to degrade dyes using the tube overlay method. Phugare ss, kalyani dc, patil av, jadhav jp 2010 textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies. Nov 20, 2014 berberine, palmatine, alizarin, purpurin, indigotin, and indirubin which were the major coloring compounds of phellodendron bark, madder, and indigo plant were thermally degraded in 100c oven in liquid dye form and also in silk dyed with five of these pigments. Growth, dye degradation and ligninolytic activity studies on zimbabwean white rot fungi.
Sixtythree local whiterot fungi were isolated from soil and wood samples on potato dextrose agar pda. The decolorization of the dye takes place either by adsorption on the microbial biomass or and enzymatic degradation. Microbial degradation of azo dye by pseudomonas spp 24. The degradation and decolourization mechanism, but with white rot mineralization of dyes is successful by certain fungi both adsorption and degradation can. Among the advanced oxidation processes, photocatalysis by semiconducting materials has become a leading technology in the field of environmental cleaning. The effects of ph, initial dye concentration, temperature, and shaking speed. Analysis of thermal degradation of mixed dye solution. White rot fungi have emerged as promising organism for treating color from wastewater9,10. Fungi offers cheaper and efficient alternative for decolorization or degradation of recalcitrant textile dyes. Pdf 38 biodegradation of azo dyes by fungi researchgate.
A degradation pathway has been proposed by champagne and ramsay 2005 in which the azo bond may be cleaved first martins et al. Huss1 phb poly3hydroxybutyric acid is a thermoplastic polyester synthesized by ralstonia eutropha and other bacteria as a form of intracellular carbon and energy storage and accumulated as inclusions in the cytoplasm of these. Pdf bioremediation of textile dye using white rot fungi. Oct 09, 2014 results of the dye biodegradation by soil fungi in this study using spectrophotometric analysis were even comparable with the percent dye decolorization exhibited by the whiterot fungus trametes versicolor and pleurotus ostreatus yao et al. Hplcdadms analysis was initially conducted on the untreated dye solution to find the retention time of the chromatogram peak, uvvisible absorption spectrum. Role of ligninolytic enzymes of white rot fungi pleurotus. Results and discussion broadspectrum of decolorization most of fungi screened for decolorization were valid for limited dyes.
Trametes villosa laccase was used for direct azo dye degradation, and the reaction products that accumulated after 72 h of incubation were analyzed. Microbial decolourization and degradation is an eco. Different methods employed in dye removal include membrane filtration, coagulation, flocculation, precipitation, floatation, adsorption, ionexchange. Microbial degradation of dyes by the fungus trichoderma harzianum, along with the effects of these dyes on this fungus during their biodegradation, was investigated using semisolid medium pda, himedia. Complete degradation of dye into nonharmful state or form is essential3. Pdf biodegradation of textile dyes, bromophenol blue and.
Biodegradation of dyes by basidiomycetes fungi using hplc and. However, contrary to the performance shown in aseptic batch tests, the application of white rot fungi in continuous bioreactors for dye wastewater treatment has been so far impeded by problems such as excessive growth of fungi causing reactorclogging zhang et al. Pdf growth, dye degradation and ligninolytic activity. Direct azo dye laccase degradation and aminophenol polymerization were performed for several days. The most widely explored fungi with regard to dye degradation are the ligninolytic fungi bumpus,1995. Analysis of bacterial degradation of azo dye congo red. Real dye containing wastewater samples were added to fungi and its effectiveness in dye color removal evaluated. Decolourization of dye wastewater by microbial methods a. The mechanism of fungal decolouration occurs from adsorption, enzymatic degradation or a combination of both. The initial recognition of white rot fungi competency in decolorization lays the foundation for its application in dye degradation.
Natural dyeing normally requires a color fixative such as alum. Thermal degradation of natural dyes and their analysis. The inhibition of fungal growth in dye containing medium was also observed during dye degradation table 1. Congo red is one of the best known and used azo dyes which has two azo bonds nn chromophore in its molecular structure. Biodegradation of textile dyes, bromophenol blue and congored. Fungi dye decolourization % incubation period days references phanerochaetechrysosporium methyl violet, congo red, acid orange, acid red 114, vat magenta, methylene blue and acid green 8898 310 29 trametes coriolus versicolour reactive blue 4 50 mgl 98 16 30. Oct 17, 2014 phugare ss, kalyani dc, patil av, jadhav jp 2010 textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies. Degradation of azo dyes by environmental microorganisms. The presence of living fungal cells, however, showed a better extent of dye degradation than that of in vitro systems. Fungi dye decolourization % incubation period days references. Once bacteria invade the system, bacteria can compete with white rot fungi for substrate and can thus inhibit fungal growth, rendering the white rot fungi system eventually ineffective for dye decolouration and degradation hai et al.
Degradation of drimaren brilliant blue the content of the flasks was removed at determined time intervals and filtered 0. Synthetic dye degradation by whiterot fungi and fungal. Sixty one dye effluent soil fungal strains were screened for their ability to degrade. Liquid chromatographymass spectrometry lcms analysis showed the formation of phenolic compounds during the dye oxidation process as well as a large amount of polymerized products that retain azo group integrity. Biodegradation of dyes using consortium of bacterial. Microbial degradation of textile effluents were critically analysed and discussed. This feature is probably due to the ability of white rot fungi. Furthermore, the fungi degraded these dyes without accumulating any phenolic compounds. Various approaches using fungalenzymatic degradation are summarized. Pdf on dec 17, 2003, john a bumpus and others published 38 biodegradation of azo dyes by fungi find, read and cite all the research you need on researchgate. Existing physicalchemical technologies for colour removal are very expensive. Decolourization and degradation of dyes in industrial.
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